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Dr. Moustapha Mbow

Source:

mbowDate of birth:  January 11, 1978 in Kaolack, Senegal
Address: Laboratoire de Bactériologie Virologie Hôpital
Aristide Le Dantec, BP 7325 Dakar Plateau, Dakar, Sénégal

E-mail: moustaphazero@yahoo.fr

Current function: Scientist officer in the Immunology unit of the Bacteriology and Virology Laboratory, Aristide Le Dantec University hospital and Medical Research Council (MRC) of the Gambia, Fajara.

2005: Pharmacy degree (medical biology option) – Cheikh Anta Diop University, Dakar, Senegal.

2004-2007: Dakar’s Hospitals Resident Internship.

2006: Biological team leader of epidemiological surveillance and national seroprevalence determination of STD/AIDS in Senegal – National Division against AIDS, USAID, and French Cooperation.

2008: University degree in Biological Retrovirology -Cheikh Anta Diop University, Dakar, Senegal.

2009: Master of Immunology and Infection – Cheikh Anta Diop University, Dakar, Senegal.

2011: Master of Fundamental and Applied Microbiology – Cheikh Anta Diop University, Dakar, Senegal.

2010-2013: PhD study on the project “The role of regulatory T cells and Th17 cells in the development of schistosoma pathology in Senegal” – Institute of Tropical Medicine of Antwerp / Leiden University Medical Center/ Cheikh Anta Diop University, Dakar, Senegal; DGDC of Belgium and EU funded project.

Since 2012: Scientific officer of the project “Impact of active tuberculosis and antiretroviral treatment on the Treg/Th17 balance during HIV-1 infection” – Institute of Tropical Medicine of Antwerp / Cheikh Anta Diop University, Dakar, Senegal;  DGDC of Belgium funded project.

Since 2013: Scientific officer in the project “Functional characteristics of effector and recall NK cellular responses and their comparison with adaptive T cell responses in HIV-vaccinated subjects and risk populations” – EDCTP funded project.

 

PUBLICATIONS (3 of 7)

  1. Mbow M., Santos N.S.S., Camara M., Niang A., Daneau G., Wade D., Diallo A.A., Ba A., Toupane M., Diakhaté M, Lèye N., Diaw P.A., Mboup S., Kestens L., Dieye T.N. HIV/TB co-infection impacts T cell activation markers but not the sub-set of regulatory T cells in HIV-1 infected patients. Af J lab Med 2013.
  2. Mbow M, Larkin BM, Meurs L, Wammes LJ, de Jong SE, Labuda LA, Camara M, Smits HH, Polman K, Dieye TN, Mboup S, Stadecker MJ, Yazdanbakhsh M. T-helper 17 cells are associated with pathology in human schistosomiasis. J Infect Dis. 2013 Jan 1;207(1):186-95.
  3. Lynn Meurs, Moustapha Mbow, Kim Vereecken, Joris Menten, Souleymane Mboup, Katja Polman. Bladder morbidity and hepatic fibrosis in mixed Schistosoma haematobium and S. mansoni Infections: a population-wide study in Northern Senegal. PLoS Negl Trop Dis. 2012;6(9):e1829.

 

 

Abstract Title

Natural Killer Cells Of HIV-1 Exposed But Uninfected Subjects Exhibit Recall Responsiveness To HIV-1 Peptides

Abstract text (max 2500 characters incl spaces):

Background: The function and phenotype of cells able to protect against HIV infection or limit HIV transmission remains unclear.

Difficulties in developing effective T cell-based vaccines against HIV highlight the urgent need for investigation of other cellular

immune correlates of protective immunity in clinical trial settings and high risk cohorts. Recent appreciation of the potential of NK

cells to contribute to antigen-specific secondary immune responses as well as to innate responses suggests that these cells deserve

more attention. Here we investigated ex-vivo effector and recall NK cell responses in HIV-1 infected adults and their HIV-exposed

but uninfected partners. Methods: We selected 10 HIV-1 positive individuals and their HIV-negative partners from a serodiscordant

cohort in Dakar, Senegal. We recruited 9 HIV-sero-negative subjects as controls. PBMCs were stimulated for 18h with

whole HIV-1 clade A peptide pools of Reg (Tat, Rev, Vif, Vpu, and Vpr), Gag, and Env 15-mers overlapping by 10 amino acids.

We used multiparametric flow cytometry to analyze NK cells including the functional markers CD107a and IFN-γ, the activation

marker CD25, the CD94/NKG2C heterodimeric activating receptor complex, and the CXCR6+ (putative “memory”) phenotype.

Results: In response to Reg peptides, a higher proportion of NK cells from HIV-exposed but uninfected partners expressed

CD107a (7.7% [IQR:6.3-8.0]) compared to their HIV-1 positive partners (4.2% [IQR:3.6-5.4], p=0.001) or controls (5.0%

[IQR:3.7-6.3], p=0.009). The proportion of NK cells producing IFN-γ in response to Reg peptides was also higher among

uninfected partners (10.4% [IQR:7.2-13.5]) than among HIV-1 positive partners (5.6% [IQR:4.1-8.7], p=0.048). Conversely, the

percentage of CD25+ NK cells and the overall proportion of CD94+NKG2C+ NK cells was significantly higher in HIV-positive

individuals (13.5% [IQR:11.2-15.6] and 6.5% [IQR:5.7-7.8] respectively) than among their uninfected partners (7.0% [IQR:4.7-

8.3], p<0.001 and 3.2% [IQR:1.2-4.6], p=0.002, respectively). Exposed uninfected subjects also displayed higher NK CD107a

responses to Gag peptides (7.1% [IQR:5.9-9.4]) than did their HIV-1 infected partners (5.1% [IQR:4.6-5.9], p=0.010) and

controls (5.4% [IQR:IQR:3.6-6.2], p=0.011). There was a trend towards a higher frequency of CXCR6+ NK cells in HIV+

subjects compared to both HIV-negative populations but this did not reach statistical significance. Conclusions: These preliminary

data suggest that exposure to HIV antigens may prime recall NK cell responses. Since such responses may be dependent on

antigen-specific IL-2 secretion from CD4+ T cells, studies are in progress to determine whether NK cell responses correlate with

Reg/Gag-specific T cell responses and whether loss of such T cells affects the NK cell response.

References

Barouch DH et al, Annu Rev Med 2010; 61:153-67. Vivier E et al, Nat Immunol 2008 May; 9(5):503-10. Smyth MJ, Mol

Immunol; 42(4):501-10 Horowitz A et al, Front Immunol 2011; 2:88. Paust S et al, Nat Immunol 2010; 11(12):1127-35.

Tiemessen CT et al, J Immunol 2009; 182(10):5914-8. Bruunsgaard H, Scand J Immunol; 46(1):91-5.

Track Descriptors

112 Track A: Immunology of HIV – A7. Immune responses in resistant cohorts: elite controllers and exposed uninfected

Authors of the Abstract

Mbow, M., Moustapha, Laboratory of Bacteriology and Virology of Aristide Le Dantec University Hospital, Dakar, Senegal.,

Senegal (Presenting); Jallow, S., Sabelle, Medical Research Council (MRC) Unit, The Gambia, Gambia (Presenting); Ndour, C.T.,

Cheikh Tidiane, Clinic of Infectious Disease of Fann University Hospital, Dakar, Senegal., Senegal; Mboup, S., Souleymane,

Laboratory of Bacteriology and Virology of Aristide Le Dantec University Hospital, Dakar, Senegal., Senegal; Goodier, M., Martin,

Department of Immunology and Infection, London School of Hygiene & Tropical Medicine, UK., United Kingdom; Riley, E.,

Eleanor, Department of Immunology and Infection, London School of Hygiene & Tropical Medicine, UK., United Kingdom; Jaye,

A., Assan, Medical Research Council (MRC) Unit, The Gambia., Laboratory of Bacteriology and Virology of Aristid, Gambia

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